VINEYARD
TABLE 2: DETECTION OF INFECTED SAMPLES BY HEALTHCHECK AND TAQMAN Virus
Tested
GLRaV-1 GLRaV-2
GLRaV-2-RG GLRaV-3 GLRaV-4 GLRaV-5 GLRaV-7 GVA GVB GVD GFkV
RSPaV
TaqMan® Detected
12 23 2
20 3 4 2
13 15 0
16 NT
HealthCheckTM Detected
14 25 2
30 6 6 2
13 19 0
24 120
Percentage TaqMan®
Missed
14% 8% 0%
33% 50% 33% 0% 0%
21% 0%
33% ND
some might claim that one meth- od is better than another, the best approach is to use many comple- mentary methods in combination. This author's experience finds
that it is detrimental to rely solely on one detection method for the production of clean planting stock or vineyard disease management. More than one complementary detection method, namely ELISA and one of the PCR methods described here, should be used for accurate and sensitive pathogen detection.
Judit Monis is the plant health ser- vices division manager at Eurofins STA Laboratories, with locations in California and Colorado. She may be contacted at (408) 846-9964 or judit- monis@eurofinsus.com.
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SEPT - OCT 2012 VINEYARD & WINERY MANAGEMENT 79
